Characterization of MPXV-GFP-tdTR. (a) Vero-E6 cells infected with MPXV-GFP-tdTR were imaged 48 h p.i. by fluorescence microscopy. A single representative plaque is shown. Green is GFP, red is Tomato Red, and yellow represents the overlap of green and red fluorescence. (b) Vero-E6 cells infected with MPXV-GFP-tdTR (MOI = 5) in the presence or absence of Ara-C were imaged after 48 h by fluorescence microscopy. Green is GFP, red is Tomato Red, and yellow represents the overlap of green and red fluorescence. (c) High MOI growth curve. HeLa cells were infected with MPXV-GFP-tdTR at an MOI = 5. Cells (left) and medium (right) were separately harvested at the indicated times, and lysates were titered by plaque assay. Note the different scales for cell and media graphs.
Johnston et al. Virology Journal 2012 9:5 doi:10.1186/1743-422X-9-5