Dose-dependent inhibitory effect of shRNA expression vector p70/GFP. Vero cells were transfected with variant p70/GFP and then infected with GTPV at an MOI of 0.01. 48 h post-infection, total RNA was extracted and subjected to quantitative PCR analysis. Mock-transfected cells were used as controls. The mRNA of beta actin served as an internal reference. Black bars indicate normalized shRNA+ (cells transfected with p70/GFP at six different concentrations ranging from 0.1 to 5.0 μg) GTPV message copies ratios. The data shown represent average from three experiments with the standard deviations indicated by error bars.
Zhao et al. Virology Journal 2012 9:48 doi:10.1186/1743-422X-9-48