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Open Access Short report

Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay

Yang Jin-Long1, Yang Rui1, Shen Ke-Fei1, Peng Xiang-Wei1, Xiong Tao2 and Liu Zuo-Hua1*

Author Affiliations

1 Chongqing Academy of Animal Science, Chongqing 402460, China

2 Rongchang Bureau of Animal Husbandry, Chongqing 402460, China

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Virology Journal 2012, 9:47  doi:10.1186/1743-422X-9-47

Published: 17 February 2012

Abstract

Background

Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required.

Findings

A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green.

Conclusions

The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.

Keywords:
Sacbrood virus; Loop-mediated isothermal amplification; SYBR Green; Honeybee