Rapid detection of sacbrood virus (SBV) by one-step reverse transcription loop-mediated isothermal amplification assay
1 Chongqing Academy of Animal Science, Chongqing 402460, China
2 Rongchang Bureau of Animal Husbandry, Chongqing 402460, China
Virology Journal 2012, 9:47 doi:10.1186/1743-422X-9-47Published: 17 February 2012
Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required.
A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green.
The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.