PcAb reactionogenicity and specificity were identified by IFA. BHK-21 cell incubated with JEV for 1 h at 37°C. At 72 h post-infection, cells were fixed with absolute methanol and treated for IFA with the prepared PcAb against JEV. Normal BHK-21 without infected JEV as a control (MOCK).
Mei et al. Virology Journal 2012 9:4 doi:10.1186/1743-422X-9-4