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Open Access Research

Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits

Jian Ben Wang1, Yali Zhu2, Michael A McVoy1* and Deborah S Parris2

Author Affiliations

1 Department of Pediatrics, Virginia Commonwealth University, P.O. Box 163, MCV Station, Richmond, VA, 23298-0163, USA

2 Department of Molecular Virology, Immunology, and Medical Genetics, Ohio State University, Columbus, OH, 43210, USA

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Virology Journal 2012, 9:315  doi:10.1186/1743-422X-9-315

Published: 21 December 2012

Abstract

Background

During herpesvirus replication, terminase packages viral DNA into capsids. The subunits of herpes simplex virus terminase, UL15, UL28, and UL33, assemble in the cytoplasm prior to nuclear import of the complex.

Methods

To detect similar interactions between human cytomegalovirus terminase subunits, the orthologous proteins UL89, UL56, and UL51 were expressed in HEK-293‚ÄČT cells (via transfection) or insect cells (via baculovirus infection) and subcellular localizations were detected by cellular fractionation and confocal microscopy.

Results

In both cell types, UL56 and UL89 expressed alone were exclusively cytoplasmic, whereas UL51 was ~50% nuclear. Both UL89 and UL56 became ~50% nuclear when expressed together, as did UL56 when expressed with UL51. Nuclear localization of each protein was greatest when all three proteins were co-expressed.

Conclusions

These results support inclusion of UL51 as an HCMV terminase subunit and suggest that nuclear import of human cytomegalovirus terminase may involve nuclear import signals that form cooperatively upon subunit associations.

Keywords:
Herpesvirus; Cytomegalovirus; Terminase; DNA packaging; Genome maturation