CHIKV positive and negative RNA strands expression. Positive (+) and negative (-) E2 RNAs were detected at very high levels in CLTT cells ( both in cells and supernatants) whereas RAW264.7 expressed the positive strand at level 1000 fold lower than CLTT (both in cells and supernatants) while the negative strand was detected at a low level and only in cell extract. The quantity of RNA strands (arbitrary unit) was estimated with the ΔCt method as 2-Δct with ΔCt = Ct (at 8 h or 48 h PI) – Ct (at T0). Ndt (Not detected).
Kumar et al. Virology Journal 2012 9:313 doi:10.1186/1743-422X-9-313