RAW264.7 infected cells does not undergo apoptosis. (A& B) Immunofluorescence of RAW264.7 and CLTT at 48 hrs PI (mouse monoclonal anti-CHIKV E1, green fluorescence) and apoptosis markers (initiator cleaved caspase-9 or executor cleaved caspase-3, red fluorescence). Nuclei were stained with DAPI (blue fluorescence). (C) No DNA fragmentation (TUNEL assay) was observed in RAW264.7 cells (Inset, positive control of apoptosis) during the time course of infection.
Kumar et al. Virology Journal 2012 9:313 doi:10.1186/1743-422X-9-313