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Open Access Short report

Inactivation of Chikungunya virus by 1,5 iodonapthyl azide

Anuj Sharma1, Paridhi Gupta12 and Radha K Maheshwari1*

Author Affiliations

1 Department of Pathology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USA

2 Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Rajasthan, India

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Virology Journal 2012, 9:301  doi:10.1186/1743-422X-9-301

Published: 4 December 2012

Abstract

Background

Chikungunya virus (CHIKV) is an arthropod borne alphavirus of the family Togaviridae. CHIKV is a reemerging virus for which there is no safe prophylactic vaccine. A live attenuated strain of CHIKV, CHIK181/25, was previously demonstrated to be highly immunogenic in humans, however, it showed residual virulence causing transient arthralgia.

Findings

In this study, we demonstrate the complete inactivation of CHIKV181/25 by 1,5 iodonapthyl azide (INA). No cytopathic effect and virus replication was observed in cells infected with the INA-inactivated CHIKV. However, a reduction in the INA-inactivated CHIK virus-antibody binding capacity was observed by western blot analysis.

Conclusion

INA completely inactivated CHIKV and can further be explored for developing an inactivated-CHIKV vaccine.

Keywords:
Chikungunya virus; Inactivated vaccine; Iodonapthyl azide