Additional file 4.

Evolutionary relationship of PA gene of Pakistani HP H5N1 viruses compared with other representative Eurasian H5N1 viruses. The nucleotide coding regions tree was generated by neighbour joining method (with Maximum Composite Likelihood) as implemented in MEGA version 4. Numbers at the nodes indicate confidence level of a bootstrap analysis with 1000 replications as a percentage value. Scale bar indicates 0.01 nucleotide substitutions per site. The Pakistani isolates are marked in dark circles. Tree is midpoint rooted.

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Siddique et al. Virology Journal 2012 9:300   doi:10.1186/1743-422X-9-300