Cytokine expression in DENV infected cells after gene silencing.A-F) mRNA levels of selective cytokines/chemokines (A:Ifnβ1, B:Il 6, C:Cxcl1,D:Cxcl2,E:Ccl5,F:Ccr5) were measured by qRT-PCR and normalized to mouse beta-actin gene. G-H) Protein level of IFNβ and IL6 in cell supernatants. Results are expressed as the mean + the SEM. * p <0.05 (t-test). Representative results from at least 3 independent experiments.
Pan et al. Virology Journal 2012 9:292 doi:10.1186/1743-422X-9-292