The viral burden is increased in Gbp1 silenced cells.A-B) RNAi efficiency for Gbp1 as shown in qRT-PCR (A) and Western blot (B). C-D) DENV burdens in RAW264.7 cells after RNAi silencing. C) The viral burdens were analyzed by measuring the virus E gene copy using qRT-PCR, and normalized to mouse beta actin gene. D) The titer of infectious DENV in supernatants of cells. Y axis represents the number of plaques formed in 293T cells when infected with viruses in 100 μl of cell supernatants 24h post infection. Results are expressed as the mean + the SEM. * p < 0.05 and ** p < 0.01 (t-test). Representative results from at least 3 independent experiments.
Pan et al. Virology Journal 2012 9:292 doi:10.1186/1743-422X-9-292