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Guanylate-binding protein 1 participates in cellular antiviral response to dengue virus

Wen Pan, Xiangyang Zuo, Tingting Feng, Xiaohong Shi and Jianfeng Dai*

Author Affiliations

Institute of Biology and Medical Sciences, Jiangsu Key Laboratory of Infection and Immunity, Soochow University, Building 703, 199 Ren-ai Road, Suzhou, 215123, P.R. China

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Virology Journal 2012, 9:292  doi:10.1186/1743-422X-9-292

Published: 27 November 2012

Abstract

Background

Dengue virus (DENV), the causative agent of human Dengue hemorrhagic fever, is a mosquito-borne virus found in tropical and sub-tropical regions around the world. Vaccines against DENV are currently unavailable. Guanylate-binding protein 1 (GBP1) is one of the Interferon (IFN) stimulated genes (ISGs) and has been shown important for host immune defense against various pathogens. However, the role of GBP1 during DENV infection remains unclarified. In this study, we evaluated the relevance of GBP1 to DENV infection in in vitro model.

Findings

Quantitative RT-PCR (qRT-PCR) and Western blot showed that the expression of mouse Gbp1 was dramatically upregulated in DENV-infected RAW264.7 cells. The intracellular DENV loads were significantly higher in Gbp1 silenced cells compared with controls. The expression levels of selective anti-viral cytokines were decreased in Gbp1 siRNA treated cells, while the transcription factor activity of NF-κB was impaired upon GBP1 silencing during infection.

Conclusions

Our data suggested that GBP1 plays an antiviral role during DENV infection.

Keywords:
GBP1; DENV; Antiviral response; NF-κB