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Open Access Highly Accessed Methodology

Evaluation of nanoparticle tracking analysis for total virus particle determination

Petra Kramberger12*, Mateja Ciringer2, Aleš Štrancar12 and Matjaž Peterka12

Author Affiliations

1 BIA Separations, d.o.o, Mirce 21, SI-5270, Ajdovščina, Slovenia

2 CO-BIK, Velika pot 22, SI-5250, Solkan, Slovenia

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Virology Journal 2012, 9:265  doi:10.1186/1743-422X-9-265

Published: 12 November 2012

Abstract

The NanoSight LM10 with Nanoparticle tracking analysis (NTA) software was evaluated for the quantification of latex particles, adenovirus 5, and influenza virus. The inter-day variability was determined by measuring the same sample over several consecutive days and the method’s accuracy was demonstrated by using known concentrations of the subject particles. NTA analysis was also used to quantify chromatographic fractions of adenovirus and influenza virus after purification on a CIM monolithic column. NTA results were compared and evaluated against hemagglutination (HA) and end point dilution assay, determining total and infection virus particle number, respectively. The results demonstrated that nanoparticle tracking analysis is a method for fast estimation of virus concentration in different samples. In addition, it can provide a better insight into the sample status, regarding the level of virus aggregation.

Keywords:
NanoSight; NTA; Adenovirus 5; Influenza; Latex particles; CIM monolithic column