Table 1

Benefits and weaknesses of the molecular methods for HPV detection
Method Benefits Weaknesses
Nucleic acids hybridization assays Southern blot is gold standard for HPV genomic analysis Presence of HPV in association with morphology Low sensitivity , time consuming, relatively large amounts of purified DNA
Southern blot and hybridization cannot use degraded DNA
Signal amplification assays Quantitative Licensed and patented technologies
FDA-approved test (hc2) Wasn’t designed to genotyping individual
Lower false-positive rate
High sensitivity to genotyping
Nucleic acids amplification assays Flexible technology (viral load and genotype) Lower amplification signals of some HPV genotypes
Very high sensitivity Contamination with previously amplified material can lead to false positives
Multiplex analysis

HPV = Human Papillomavirus; FDA = Food and Drug Administration; hc2 = Hybrid Capture® 2; PCR = Polymerase Chain Reaction.

Abreu et al.

Abreu et al. Virology Journal 2012 9:262   doi:10.1186/1743-422X-9-262

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