|Benefits and weaknesses of the molecular methods for HPV detection|
|Nucleic acids hybridization assays||Southern blot is gold standard for HPV genomic analysis Presence of HPV in association with morphology||Low sensitivity , time consuming, relatively large amounts of purified DNA|
|Southern blot and hybridization cannot use degraded DNA|
|Signal amplification assays||Quantitative||Licensed and patented technologies|
|FDA-approved test (hc2)||Wasn’t designed to genotyping individual|
|Lower false-positive rate|
|High sensitivity to genotyping|
|Nucleic acids amplification assays||Flexible technology (viral load and genotype)||Lower amplification signals of some HPV genotypes|
|Very high sensitivity||Contamination with previously amplified material can lead to false positives|
HPV = Human Papillomavirus; FDA = Food and Drug Administration; hc2 = Hybrid Capture® 2; PCR = Polymerase Chain Reaction.
Abreu et al.
Abreu et al. Virology Journal 2012 9:262 doi:10.1186/1743-422X-9-262