HCMV infection alters expression of CXCR4 and CXCR7. SGHPL-4 cells were mock-infected or infected with HCMV, and then CXCR4 and CXCR7 receptor expression was determined. HCMV infection induced upregulation of cell surface staining for CXCR4 (A) and CXCR7 (B) at all time points studied. (*, p<0.05; **, p<0.01; ***, p<0.001). Total RNA was harvested from mock-infected or HCMV-infected SGHPL-4 cells, and analyzed by real-time PCR using primers specific for CXCR4 and CXCR7. HCMV infection induced a significant upregulation of CXCR4 mRNA expression (C) at 8, 24, and 48 hours post-infection. A modest but significant upregulation of CXCR7 mRNA expression was seen at 48 hours post-infection (D). Protein lysates from mock-infected and HCMV-infected cells were analyzed by Western blot for CXCR4 and CXCR7 expression. No differences in absolute protein levels were seen at any time point analyzed (E).
Warner et al. Virology Journal 2012 9:255 doi:10.1186/1743-422X-9-255