HCMV infection decreases secretion of CXCL12 by SGHPL-4 cells but mRNA expression is unaffected. Supernatants were collected from mock-infected or HCMV-infected cells and CXCL12 secretion was analyzed by ELISA (A). At all timepoints studied, HCMV-infected cells secreted significantly less CXCL12 into their culture medium as compared to mock-infected controls. RNA was isolated from mock-infected and HCMV-infected cells and quantitative real-time PCR was performed using primers specific for CXCL12 (B). There was no significant difference in mRNA expression between mock-infected and HCMV-infected cells at any timepoint analyzed. (*, p<0.05; **, p<0.01; ***, p<0.001).
Warner et al. Virology Journal 2012 9:255 doi:10.1186/1743-422X-9-255