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Molecular detection of a novel paramyxovirus in fruit bats from Indonesia

Michihito Sasaki1, Agus Setiyono3, Ekowati Handharyani3, Ibenu Rahmadani4, Siswatiana Taha5, Sri Adiani6, Mawar Subangkit3, Hirofumi Sawa1, Ichiro Nakamura2 and Takashi Kimura1*

Author Affiliations

1 Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

2 Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan

3 Laboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia

4 Veterinary Investigation and Diagnostic Center, Bukittinggi, Indonesia

5 Faculty of Agriculture, Gorontalo State University, Gorontalo, Indonesia

6 Faculty of Animal Husbandry, Sam Ratulangi University, Manado, Indonesia

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Virology Journal 2012, 9:240  doi:10.1186/1743-422X-9-240

Published: 19 October 2012

Abstract

Background

Fruit bats are known to harbor zoonotic paramyxoviruses including Nipah, Hendra, and Menangle viruses. The aim of this study was to detect the presence of paramyxovirus RNA in fruit bats from Indonesia.

Methods

RNA samples were obtained from the spleens of 110 fruit bats collected from four locations in Indonesia. All samples were screened by semi-nested broad spectrum reverse transcription PCR targeting the paramyxovirus polymerase (L) genes.

Results

Semi-nested reverse transcription PCR detected five previously unidentified paramyxoviruses from six fruit bats. Phylogenetic analysis showed that these virus sequences were related to henipavirus or rubulavirus.

Conclusions

This study indicates the presence of novel paramyxoviruses among fruit bat populations in Indonesia.