Table 1

Sequences of primers used for the PCRs
Primers Sequences (5'-3') Underlined restriction site Position on MYVB
Y47βp138-F
    GAATTCAAGCTT
CTCCATCATATCTTTAAAGT
EcoRI, HindIII 690-671
Y47βp214-F
    GAATTCAAGCTT
CTCATGTGTACGTATATATACG
EcoRI, HindIII 766-745
Y47βp267-F
    GAATTCAAGCTT
CGTTTTCTTTCCTTTTCAGTTCAG
EcoRI, HindIII 819-796
Y47βp389-F
    GAATTCAAGCTT
CAAACGACATCGTTTTCGAGTT
EcoRI, HindIII 941-920
Y47βp418-F
    GAATTCAAGCTT
TTATTATTTTTTTCTTTTTTTCTTC
EcoRI, HindIII 971-947
Y47βp-F
    GAATTCAAGCTT
TTTCAAATAACAGATCATCAAG
EcoRI, HindIII 195-174
Y47βp-R
    GAGCTCGGATCC
CTTGCTTGTGTGATGGAAGTG
SacI, BamHI 553-573
Y47βΔUTR-F TTTTCTTTTTCTCAAACGACATCGTTTTCG 962-953 plus 943-924
Y47βΔUTR-R GTCGTTTGAGAAAAAGAAAAAAATAATAATGGGG 934-943 plus 953-976
β01 GTA
    GGTACC
ACTACGCTACGCAGCAGCC
KpnI 1290-1308
β02 AGT
    GGTACC
TACCCTCCCAGGGGTACAC
KpnI 1283-1265
β03 GTAGAAACCACTACGCTACGCAGCAGCC 1290-1308

Zhang et al.

Zhang et al. Virology Journal 2012 9:234   doi:10.1186/1743-422X-9-234

Open Data