Neutralization of IL-22 increased the productions of IL-17, TNF-α and IL-6, decreased the level of IFN-γ, promoted cardiac viral replication on day 14 post infection. Relative cardiac expressions of IL-17, TNF-α, IL-6, IFN-γ and IL-1β were investigated by RT-PCR, and the levels of their circulating protein were measured by ELISA in Anti-IL-22 Ab, IgG control and PBS groups. A-E: IL-17, IL-6, TNF-α, IFN-γ and IL-1β, respectivity. F. The cardiac CVB3 titers and relative expressions of CVB3 RNA were investigated by PFU and RT-PCR. *p < 0.05, **p < 0.01. Data are mean ± SD.
Kong et al. Virology Journal 2012 9:232 doi:10.1186/1743-422X-9-232