Table 1

Characterization of the fluorescent molecules used to tag HCMV tegument proteins pp65, pp71, pp150, and pp28

Fluorescent Molecule

Tegument Protein(s) Labeled

Excitation Peak (nm)

Emission Peak (nm)

Brightness

Photostability

pKa


Cyan

pp65

433/445

475/503

13

64

4.7


RFP

pp71

584

607

12.5

8.7

4.5


GFP

pp150 and pp28

488

507

34

174

6.0


Tomtishen III Virology Journal 2012 9:22   doi:10.1186/1743-422X-9-22

Open Data