The rabbit astrovirus genome. (A) A cartoon showing the organization of the viral genome. Scale bar indicates nucleotide position. The location of a putative ribosomal frameshift motif is indicated by an arrowhead. The position of the two overlapping Virochip probes that were originally positive for an astrovirus are indicated. (B) Location of overlapping PCR, 5′, and 3′ RACE amplicons used to determine and confirm the sequence of the viral genome. (C) A histogram showing the number of Ion Torrent reads (x1000 reads) aligning at each nucleotide position in the genome. (D) The average pairwise percent identity between the predicted rabbit astrovirus protein sequences and the sequences of the proteins from the astroviruses shown in Figure 3. The histogram is binned into 20 amino acid windows.
Stenglein et al. Virology Journal 2012 9:216 doi:10.1186/1743-422X-9-216