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Multiple linear epitopes (B-cell, CTL and Th) of JEV expressed in recombinant MVA as multiple epitope vaccine induces a protective immune response

Fengjuan Wang1, Xiuli Feng1, Qisheng Zheng2, Hongyan Hou3, Ruibing Cao1*, Bin Zhou1, Qingtao Liu4, Xiaodong Liu1, Ran Pang1, Jin Zhao1, Wenlei Deng1 and Puyan Chen1

  • * Corresponding author: Ruibing Cao crb@njau.edu.cn

  • † Equal contributors

Author Affiliations

1 Key Laboratory of Animal Diseases Diagnosis and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China

2 National Veterinary Biological Medicine Engineering Research Center, Nanjing, 210014, People's Republic of China

3 Veterinary Office, Gansu Agriculture and Animal Husbandry Department, Lanzhou, 730000, People's Republic of China

4 Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China

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Virology Journal 2012, 9:204  doi:10.1186/1743-422X-9-204

Published: 17 September 2012

Abstract

Epitope-based vaccination might play an important role in the protective immunity against Japanese encephalitis virus (JEV) infection. The purpose of the study is to evaluate the immune characteristics of recombinant MVA carrying multi-epitope gene of JEV (rMVA-mep). The synthetic gene containing critical epitopes (B-cell, CTL and Th) of JEV was cloned into the eukaryotic expression vector pGEM-K1L, and the rMVA-mep was prepared. BALB/c mice were immunized with different dosages of purified rMVA-mep and the immune responses were determined in the form of protective response against JEV, antibodies titers (IgG1 and IgG2a), spleen cell lymphocyte proliferation, and the levels of interferon-γ and interleukin-4 cytokines. The results showed that live rMVA-mep elicited strongly immune responses in dose-dependent manner, and the highest level of immune responses was observed from the groups immunized with 107 TCID50 rMVA-mep among the experimental three concentrations. There were almost no difference of cytokines and neutralizing antibody titers among 107 TCID50 rMVA-mep, recombinant ED3 and inactivated JEV vaccine. It was noteworthy that rMVA-mep vaccination potentiates the Th1 and Th2-type immune responses in dose-dependent manner, and was sufficient to protect the mice survival against lethal JEV challenge. These findings demonstrated that rMVA-mep can produce adequate humoral and cellular immune responses, and protection in mice, which suggested that rMVA-mep might be an attractive candidate vaccine for preventing JEV infection.

Keywords:
Japanese encephalitis virus; rMVA-mep; Immune response; Protection response