Identification of B cell epitopes reactive to human papillomavirus type-16L1- derived peptides
1 Department of Obstetrics and Gynecology, Kurume University School of Medicine, Kurume, Japan
2 Department of Immunology and Immunotherapy, Kurume University School of Medicine, Kurume, Japan
3 Biostatistics Center, Kurume University, Kurume, Japan
4 Department of Obstetrics and Gynecology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan
Virology Journal 2012, 9:199 doi:10.1186/1743-422X-9-199Published: 14 September 2012
Persistent infection of human papillomavirus (HPV) types 16 and 18 causes cervical cancer. To better understand immune responses to the prophylactic vaccine, HPV 16/18 L1 virus-like particles (HPV-VLPs), we investigated B cell epitopes of HPV16 L1-derived peptides.
Sera from mice immunized with HPV-16/18 L1 VLPs were analyzed for their IgG titers against 10 different HPV16 L1-derived peptides (20-mer) that contain human leukocyte antigen (HLA)-class I A-2, A-24 and class II DR.
One 20-mer peptide at positions 300 to 319 was identified as a common B cell epitope in both Balb/c (H-2d) and C57BL/6 (H-2b) mice. Mapping analysis showed that the 10-amino-acid sequence at positions 304to 313 was an immunogenic portion. It is of note that the binding capability of this 10-mer peptide to the HLA-A2 and HLA-A24 molecules was confirmed by the HLA class I stabilization assay. In addition, one unique 20-mer was determined as a B cell epitope in each strain.
These results might provide new information for better understanding of immune responses to HPV 16 L1.