Table 1

Comparison of RCV blocking ELISA (bELISA) and competition ELISA (cELISA)
Sample ID Inhibition at dilution of 1:10 (%)a

RCV

bELISA titre

RCV

cELISAb

titre

RHDV

cELISAbtitre

RCV-1c 57 160 640 0
RCV-2 56 160 160 0
RCV-3 65 160 40 0
RCV-4 60 320 160 80
RCV-8 42 40 80 0
RCV-9 62 160 320 0
RCV-10 56
    160
    0
0
RCV-11 72 160 40 0
RCV-12 63 160 20 0
RCV-13 50
    160
    0
0
RCV-14 51
    160
    0
0
RCV-15 53
    160
    0
0
RCV-16 71 160 640 0
RCV-17 59 80 640 0
RCV-18 70 160 80 0
RCV-19 61 160 80 0
RCV-20 43
    40
    0
0
RCV-21 55
    40
    0
0
RCV-22 58 40 640 0
RCV-23 66 160 160 0
RCV-24 74 320 160 0
RCV-25 66 160 320 0
RHDV-1 6 0 0 320
RHDV-2 0 0 0 81,960
RHDV-3 0 0 0 20,480
RHDV-4 10 0 0 640
RHDV-5 0 0 0 160
RHDV-6 0 0 0 160
RHDV-7 2 0 0 160
RHDV-8 2 0 0 320
RHDV-9 2 0 0 640
RHDV-W1d 7 0 0 80
RHDV-W2 1 0 0 80
RHDV-W3 1 0 0 320
RHDV-W4 1 0 0 80
RHDV-W5 1 0 0 160
RHDV-W6 0 0 0 160
RHDV-W7 6 0 0 80
RHDV-W8 6 0 0 320
RHDV-W9 5 0 0 80
RHDV-W10 8 0 0 40
RHDV-W11 7 0 0 40
RHDV-W12 0 0 0 160
RHDV-W13 0 0 0 640
RHDV-W14 3 0 0 320
RHDV-W15 12 0 0 320
RHDV-W16 6 0 0 320
RHDV-W17 13 0 0 160
RHDV-W18 12 0 0 320
RHDV-W19 23 0 0 640
RHDV-W20 7 0 0 80
RHDV-W21 20 0 0 320
RHDV-W22 14 0 0 640
RHDV-W23 22 0 0 320
RHDV-W24 8 0 0 320
RHDV-W25 12 0 0 320

aThe inhibition of sera tested at dilution of 1:10 in the blocking ELISA when RCV-VLP was used at 2.5 μg/ml (1:1500) and 0.33 μg/ml (1:4000), respectively. bRCV cELISA and RHDV cELISA were performed according to the RCV and RHDV cELISA-2 in Liu et al. [25]. cData for RCV cELISA and RHDV cELISA of sera RCV-1 to RHDV-9 are from Liu et al. [25] for comparison. dSera RHDV-W1 to W25 were sampled from wild rabbit populations proven to be free of RCV-A1 by quantitative PCR and serological tests.

Liu et al.

Liu et al. Virology Journal 2012 9:182   doi:10.1186/1743-422X-9-182

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