Design of the blocking ELISA. (A) The five components of the assay are added in succession according to their number. Anti-RCV chicken IgY is used for coating; (B) Negative result: negative rabbit serum does not bind to RCV-VLP and shows no inhibition, so the monoclonal antibody gives 100% signal; (C) Positive result: RCV specific antibodies in rabbit serum bind to the same epitope as the RCV-A1 specific mAb, resulting in inhibition of the signal.
Liu et al. Virology Journal 2012 9:182 doi:10.1186/1743-422X-9-182