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In vitro infection with classical swine fever virus inhibits the transcription of immune response genes

Li Feng12, Xiao-Quan Li2, Xiao-ning Li12, Jun Li12, Xian-Ming Meng2, Hong-Yun Zhang2, Jing-Jing Liang2, Hui Li2, Shi-Kai Sun2, Xin-Bin Cai2, Li-Juan Su2, Shan Yin2, Yan-Sheng Li2 and Ting Rong Luo12*

Author Affiliations

1 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, China

2 College of Animal Sciences and Veterinary Medicine, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, China

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Virology Journal 2012, 9:175  doi:10.1186/1743-422X-9-175

Published: 28 August 2012



Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear.


In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p<0.001).


We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF.

Classical swine fever virus (CSFV); Immune response genes; Real-time RT-PCR