Figure 6.

Effect of agents that inhibit endocytic virion uptake or low pH-dependent infection. (A) CHO-A and Vero cells were incubated with KOS/tk12 (MOI = 3) in hypertonic medium containing 0.3 M sucrose for 1 h at 4°C and infections were performed in the continuous presence of sucrose followed by ONPG assay. (B) CHO-K1 cells were incubated with KOS/tk12 (MOI = 3) in hypertonic medium containing 0.3 M sucrose for 1 h at 4°C, sHA102 was added to 500 nM, and infections were performed in the continued presence of sucrose followed by ONPG assay. (C, D) CHO-K1 cells and CHO-HVEM-12 cells (CHO-A) were pretreated with the indicated amounts of NH4Cl (C) or monensin (D) for 30 min at 4°C prior to KOS/tk12 attachment at 4°C (MOI = 3), addition of sHA102 to 500 nM (CHO-K1 cells only), and infection as in previous figures in the continued presence of the respective drugs. Infection was measured by ONPG assay. Values are averages of triplicate determinations.

Baek et al. Virology Journal 2012 9:15   doi:10.1186/1743-422X-9-15
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