High level activity of the KDR promoter is specific to endothelial cells. A. The luciferase reporter construct pGL3-KDR was transfected into ECV304 and HepG2 cells. The transfection efficiency was corrected by cotransfection with pSV-βgal. Results are expressed as a percentage of pGL3-SV40 Control activity for each cell type. B. The amplification of KDR promoter and CDglyTK fusion gene from recombinant viruses. 1: λ-EclT14I DNA marker; 2: Negative control; 3 and 4: The PCR products of CDglyTK gene and KDR promoter using the recombinant viruses as the template. C. Expression of recombinant TK and CD protein in the infected and uninfected cells analyzed by Western blotting.
Jia et al. Virology Journal 2011 8:74 doi:10.1186/1743-422X-8-74