Email updates

Keep up to date with the latest news and content from Virology Journal and BioMed Central.

Open Access Open Badges Research

Double suicide genes selectively kill human umbilical vein endothelial cells

Weiguo Jia, Longyong Mei, Yanping Wang, Lunxu Liu and Guowei Che*

Virology Journal 2011, 8:74  doi:10.1186/1743-422X-8-74

PubMed Commons is an experimental system of commenting on PubMed abstracts, introduced in October 2013. Comments are displayed on the abstract page, but during the initial closed pilot, only registered users can read or post comments. Any researcher who is listed as an author of an article indexed by PubMed is entitled to participate in the pilot. If you would like to participate and need an invitation, please email, giving the PubMed ID of an article on which you are an author. For more information, see the PubMed Commons FAQ.

Is ECV304 an endothelial cell line?

Masanori Terajima   (2012-03-30 09:52)  University of Massachusetts Medical School

I am surprised to see that ECV304 cell line is still being used as an endothelial cell line after two papers in 1999 and 2000 clearly showed that it is not.

The two papers are

1: Dirks WG, MacLeod RA, Drexler HG. ECV304 (endothelial) is really T24 (bladder carcinoma): cell line cross- contamination at source. In Vitro Cell Dev Biol Anim. 1999 Nov-Dec;35(10):558-9. PubMed PMID: 10614862.

2: Brown J, Reading SJ, Jones S, Fitchett CJ, Howl J, Martin A, Longland CL, Michelangeli F, Dubrova YE, Brown CA. Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83. Lab Invest. 2000 Jan;80(1):37-45. PubMed PMID: 10653001.

Competing interests

None declared


Post a comment