Table 2

Primers for cloning PCR

Target genes*

Primer code

Primers Sequences (5'-3')**

Fragment length

PCR condition


BoIL-2

BoIL-2 F

5' GAA

    GGA TCC
CAC CTC CTA CTT CAA GCT CTA CG 3'

405 bp

94°C for 60 s, 62°C for 60 s and 72°C for 60 s, 35 cycles

BoIL-2 R

5' CTA

    GAA TTC
CAA GTC ATT GTT GAG TAG ATG C 3'

BoGM-CSF

BoGM-CSF F

5' CTA

    GAA TTC
GCA CCT ACT CGC CCA CCC AA 3'

378 bp

94°C for 60 s, 62°C for 60 s and 72°C for 60 s, 35 cycles

BoGM-CSF R

5' TTA

    CCG CGG
CTT CTG GGC TGG TTC CCA G 3'

VP1

VP1 F

5'GCA

    CCG CGG
ACC ACC TCT GCG GGT GAG TCT 3'

669 bp

94°C for 60 s, 61°C for 60 s and 72°C for 60 s, 35 cycles

VP1 R

5'GAC

    CTC GAG
CAG AAG CTG TTT TGC GGG T 3'

VP1 F1

5'GCA

    GAA TTC
ACC ACC TCT GCG GGT GAG TCT 3'

VP1 R1

5'GAC

    CTC GAG
CAG AAG CTG TTT TGC GGG T 3'


Note: *The GeneBank accession number: BoIL-2 (NM_180997), BoIL-6 (NM_173923), BoGM-CSF (U22385). ** In these primers, sequences for the restriction sites were indicated by underlines.

Zhang et al. Virology Journal 2011 8:7   doi:10.1186/1743-422X-8-7

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