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Open Access Highly Accessed Methodology

Higher titers of some H5N1 and recent human H1N1 and H3N2 influenza viruses in Mv1 Lu vs. MDCK cells

Sara B Hamilton1, Diane E Wyatt2, Brett T Wahlgren3, Maureen K O'Dowd1, Jane M Morrissey1, Deirdre E Daniels1 and John A Lednicky4*

Author Affiliations

1 Energy and Life Sciences Division, Midwest Research Institute, 425 Volker Boulevard, Kansas City, Missouri 64110, USA

2 KC Bio, LLC, 2111 E. Santa Fe, Suite 312, Olathe, KS 66062, USA

3 University of Kansas Medical Center, 3901 Rainbow Blvd. MS 1018, Kansas City, KS 66103, USA

4 Environmental and Global Health Department, College of Public Health and Health Professions, University of Florida at Gainesville, Box 100188, Gainesville, FL 32610, USA

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Virology Journal 2011, 8:66  doi:10.1186/1743-422X-8-66

Published: 11 February 2011



The infectivity of influenza A viruses can differ among the various primary cells and continuous cell lines used for such measurements. Over many years, we observed that all things equal, the cytopathic effects caused by influenza A subtype H1N1, H3N2, and H5N1 viruses were often detected earlier in a mink lung epithelial cell line (Mv1 Lu) than in MDCK cells. We asked whether virus yields as measured by the 50% tissue culture infectious dose and plaque forming titer also differed in MDCK and Mv1 Lu cells infected by the same influenza virus subtypes.


The 50% tissue culture infectious dose and plaque forming titer of many influenza A subtype H1N1, H3N2, and H5N1 viruses was higher in Mv1 Lu than in MDCK cells.


The yields of influenza subtype H1N1, H3N2, and H5N1 viruses can be higher in Mv1 Lu cells than in MDCK cells.