Figure 4.

Anti-adsorption activity of flavonoids against DENV-2. Flavonoids were added directly to virus inocula for 2 h at 37 C. The inocula were used to infect Vero cell monolayers in 24 wells cell culture microplates. The reduction in foci forming unit was calculated relative to the controls maintained in parallel (a) and the respective DENV-2 RNA copy numbers were quantified using qRT-PCR (b). Data from triplicate experiments were plotted using Graph Pad Prism Version 5 (Graph Pad Software Inc., San Diego, CA).