The interaction between the PARP10 protein and the NS1 protein of H5N1 AIV and its effect on virus replication
- Equal contributors
1 Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, People's Republic of China
2 Institute of Chemical Defence, Beijing 102205, People's Republic of China
3 Institute of Disease Control and Prevention, Chinese Academy of Military Medical Sciences, Beijing, People's Republic of China
4 Beijing Institute for Neuroscience, Capital Medical University, Beijing 100069, China
Virology Journal 2011, 8:546 doi:10.1186/1743-422X-8-546Published: 16 December 2011
During the process that AIV infect hosts, the NS1 protein can act on hosts, change corresponding signal pathways, promote the translation of virus proteins and result in virus replication.
In our study, we found that PARP domain and Glu-rich region of PARP10 interacted with NS1, and the presence of NS1 could induce PARP10 migrate from cytoplasm to nucleus. NS1 high expression could reduce the endogenous PARP10 expression. Cell cycle analysis showed that with inhibited PARP10 expression, NS1 could induce cell arrest in G2-M stage, and the percentage of cells in G2-M stage rise from the previous 10%-45%, consistent with the cell proliferation result. Plague forming unit measurement showed that inhibited PARP10 expression could help virus replication.
In a word, our results showed that NS1 acts on host cells and PARP10 plays a regulating role in virus replication.