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Open Access Highly Accessed Research

Mimotopes selected with neutralizing antibodies against multiple subtypes of influenza A

Yanwei Zhong1*, Jiong Cai2, Chuanfu Zhang3, Xiaoyan Xing1, Enqiang Qin1, Jing He1, Panyong Mao1, Jun Cheng4, Kun Liu3, Dongping Xu1* and Hongbin Song3*

Author Affiliations

1 Pediatric Liver Disease Research Laboratory, Institute of Infectious Diseases, Beijing 302 Hospital, Beijing, China

2 Department of Nuclear Medicine, PUMC Hospital, PUMC & CAMS, Beijing, China

3 Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, China

4 Liver Center, Beijing Ditan Hospital, Capital Medical University, Beijing, China

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Virology Journal 2011, 8:542  doi:10.1186/1743-422X-8-542

Published: 15 December 2011

Abstract

Background

The mimotopes of viruses are considered as the good targets for vaccine design. We prepared mimotopes against multiple subtypes of influenza A and evaluate their immune responses in flu virus challenged Balb/c mice.

Methods

The mimotopes of influenza A including pandemic H1N1, H3N2, H2N2 and H1N1 swine-origin influenza virus were screened by peptide phage display libraries, respectively. These mimotopes were engineered in one protein as multi- epitopes in Escherichia coli (E. coli) and purified. Balb/c mice were immunized using the multi-mimotopes protein and specific antibody responses were analyzed using hemagglutination inhibition (HI) assay and enzyme-linked immunosorbent assay (ELISA). The lung inflammation level was evaluated by hematoxylin and eosin (HE).

Results

Linear heptopeptide and dodecapeptide mimotopes were obtained for these influenza virus. The recombinant multi-mimotopes protein was a 73 kDa fusion protein. Comparing immunized infected groups with unimmunized infected subsets, significant differences were observed in the body weight loss and survival rate. The antiserum contained higher HI Ab titer against H1N1 virus and the lung inflammation level were significantly decreased in immunized infected groups.

Conclusions

Phage-displayed mimotopes against multiple subtypes of influenza A were accessible to the mouse immune system and triggered a humoral response to above virus.

Keywords:
Influenza; Mimotopes; Phage display; Vaccination; Virus challenge