Detection of A3G in prostate cancer cells of epithelial origin A3G was detected using two different polyclonal rabbit sera obtained from the NIH AIDS Reagent Program. Anti-ApoC17 was raised against a synthetic peptide comprising of the 17 C-terminal residues of A3G (Cat. No. 10082), while the other was (Anti-ApoC29) Anti-A3G C-terminal antisera raised against a C-terminal peptide representing the last 29 amino acids of human A3G coupled to a hapten (Cat. No 10201). As a loading control β-actin (Sigma Co., USA) was used. For immunoblot analysis, cell lysates (5 μg) were subjected to SDS-polyacrylamide gel electrophoresis and western blot analysis with the appropriate antibodies. Western blot analysis using, (A) Anti-ApoC29 and (B) Anti-ApoC17.
Dey et al. Virology Journal 2011 8:531 doi:10.1186/1743-422X-8-531