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Open Access Methodology

Selection of recombinant MVA by rescue of the essential D4R gene

Patricia S Ricci1, Birgit Schäfer1, Thomas R Kreil2, Falko G Falkner1 and Georg W Holzer1*

Author Affiliations

1 Baxter BioScience, Biomedical Research Center, Uferstrasse 15, 2304 Orth an der Donau, Austria

2 Baxter BioScience, 1221 Vienna, Austria

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Virology Journal 2011, 8:529  doi:10.1186/1743-422X-8-529

Published: 12 December 2011

Abstract

Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.

Keywords:
Recombinant vaccines; Uracil-DNA glycosylase; Vaccinia virus; Cell Line; Transformed; Defective viruses/genetics; Defective viruses/growth & development