Selection of recombinant MVA by rescue of the essential D4R gene
- Equal contributors
1 Baxter BioScience, Biomedical Research Center, Uferstrasse 15, 2304 Orth an der Donau, Austria
2 Baxter BioScience, 1221 Vienna, Austria
Virology Journal 2011, 8:529 doi:10.1186/1743-422X-8-529Published: 12 December 2011
Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.