Figure 4.

Western Blot analysis of the culture using H1N1 HA specific antibodies confirming the secreted expression of recombinant HA protein supernatants under denaturing and native conditions. Panel 4A: Under denaturing condition: Lane M: Pre-stained Protein marker (Fermentas, USA, #SM 0671) Lane 1: Cell culture supernatant from Pichia multiple copy integrant Clone A Lane 2: Cell culture supernatant from Pichia multiple copy integrant Clone B Lane 3: Cell culture supernatant from negative clone Panel 4B: Under native condition: Lane M: Pre-stained Protein marker (Fermentas, USA, #SM 0671) Lane 1: Cell culture supernatant from Pichia multiple copy integrant Clone A Lane 2: Cell culture supernatant from Pichia multiple copy integrant Clone B Lane 3: Cell culture supernatant from negative clone.