Research
The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine
1 Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, Rondebosch, Cape Town, 7701 South Africa
2 Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, 7925 South Africa
3 Department of Medicine, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, 7925 South Africa
4 Department of Pharmacology and Toxicology & James Graham Brown Cancer Center, University of Louisville School of Medicine, 505 South Hancock Street, Louisville KY 40202, USA
5 National Health Laboratory Service, Groote Schuur Hospital, Observatory, Cape Town, 7925 South Africa
Virology Journal 2011, 8:51 doi:10.1186/1743-422X-8-51
Published: 7 February 2011Additional files
Additional file 1:
Confirmation of GrttnC expression by pTHGrttnC derived plasmids carrying PCV-1 DNA inserts. Extracts from HEK293 cells transfected with pTHgrttnC-based plasmids (loading volumes not adjusted for transfection efficiency) were separated on 10% polyacrylamide, electroblotted onto nitrocellulose and probed for the RT component of GrttnC with RT specific antiserum (ARP 428). Lane 1. Precision Plus Kaleidoscope MWt marker. Lane 2. Positive control protein = 200 ng HIV-1HXB2 Reverse Transcriptase dimer (51 kDa, 66 kDa). Lane 3. pTHCap (empty vector). Lane 4. pTHgrttnC. Lane 5. pTHCapgrttnC. Lane 6. pTHPcapgrttnC. Lane 7. pTHPcapRgrttnC.
Format: PPT Size: 77KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
