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Open Access Research

The porcine circovirus type 1 capsid gene promoter improves antigen expression and immunogenicity in a HIV-1 plasmid vaccine

Fiona L Tanzer12, Enid G Shephard23, Kenneth E Palmer4, Marieta Burger1, Anna-Lise Williamson25 and Edward P Rybicki12*

Author Affiliations

1 Department of Molecular and Cell Biology, Faculty of Science, University of Cape Town, Rondebosch, Cape Town, 7701 South Africa

2 Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, 7925 South Africa

3 Department of Medicine, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, 7925 South Africa

4 Department of Pharmacology and Toxicology & James Graham Brown Cancer Center, University of Louisville School of Medicine, 505 South Hancock Street, Louisville KY 40202, USA

5 National Health Laboratory Service, Groote Schuur Hospital, Observatory, Cape Town, 7925 South Africa

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Virology Journal 2011, 8:51  doi:10.1186/1743-422X-8-51

Published: 7 February 2011

Additional files

Additional file 1:

Confirmation of GrttnC expression by pTHGrttnC derived plasmids carrying PCV-1 DNA inserts. Extracts from HEK293 cells transfected with pTHgrttnC-based plasmids (loading volumes not adjusted for transfection efficiency) were separated on 10% polyacrylamide, electroblotted onto nitrocellulose and probed for the RT component of GrttnC with RT specific antiserum (ARP 428). Lane 1. Precision Plus Kaleidoscope MWt marker. Lane 2. Positive control protein = 200 ng HIV-1HXB2 Reverse Transcriptase dimer (51 kDa, 66 kDa). Lane 3. pTHCap (empty vector). Lane 4. pTHgrttnC. Lane 5. pTHCapgrttnC. Lane 6. pTHPcapgrttnC. Lane 7. pTHPcapRgrttnC.

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