Dose-dependent inhibition on specific H-PRRSV gene expression by amirGP5-370 and amirM-263-M-263 was observed by qPCR. The mRNA of β-actin was served as an internal reference. GP5 and M protein coding genes expression normalized to β-actin gene in negative amiRNA transfected cells group was set at 100%. Data are mean ± SD, n = 3~4, *p < 0.05 vs. negative amiRNAs alone transfected cells group.
Xiao et al. Virology Journal 2011 8:491 doi:10.1186/1743-422X-8-491