Western blot analysis of US11 with anti-US11 polyclonal antibody. Lane 1, the lysate of HEK293T cells transfected with plasmid encoding full-length US11-HA protein; Lane 2, total protein from E. coli BL21 (DE3); Lane 3, total protein from E. coli BL21 (DE3) harboring the plasmid pET-32a-US11 after induction by IPTG; Lane 4, the lysate of mock infected Vero cells; Lane 5, the lysate of Vero cells infected with HSV-1; Lane M, the protein maker (kDa). Arrowheads indicate the position of the US11 protein.
Huang et al. Virology Journal 2011 8:490 doi:10.1186/1743-422X-8-490