Quality control of fractionation and obtained quantitative data. (A) Proteins were separated by SDS-PAGE and visualized by subsequent silver staining. SDS-PAGE analysis of the offgel fractions shows successful separation of protein on the pH strip and indicates differences in the proteome of infected versus uninfected cells. (B) All obtained logarithmic protein expression ratios, ln (R), were plotted in ascending order for each identified protein (protein id) along the x-axis. Dotted horizontal bars indicate the chosen cutoff for up- and down-regulation during RSV infection used in IPA analysis, respectively.
Ternette et al. Virology Journal 2011 8:442 doi:10.1186/1743-422X-8-442