Sialic acid dependent infection by TGEV PUR46. The infectivity of parental virus (left columns) and the HAD3 mutant virus was determined for adsorption times of 5, 20 or 60 min, respectively. The m8 and m10 mutant viruses were analyzed for adsorption times of 5 and 60 minutes. Prior to infection, ST cells were incubated for 60 min with either PBS (grey columns) or PBS containing 50 mU of neuraminidase (white columns). All experiments were performed 4 times with standard deviations shown at the corresponding columns. Significant differences are marked with an asterisk (*, p-value < 0.05).
Schwegmann-Weßels et al. Virology Journal 2011 8:435 doi:10.1186/1743-422X-8-435