U-87 MG displays a localization pattern of ZsGreen-tagged Vpr similar to U-373 MG astroglial cells. ZsGreen protein was uniformly distributed intracellularly. ZsGreen-Vpr (B) and Vpr-ZsGreen (C) localized at the nuclear envelope, along with a speckled formation intranuclearly. An accumulation around the nucleolar rim could also be observed, as well as localized herniations at the nuclear envelope. A conspicuous amount was also noticeable at the immediate outer layer of the nuclear envelope, possibly coinciding with the endoplasmic reticulum. The first, second, and third columns represent DAPI, FITC, and merged views, respectively. All figures denote a cross-sectional view of the cell and are representative of the entire cell population.
Ferrucci et al. Virology Journal 2011 8:397 doi:10.1186/1743-422X-8-397