In U-373 MG astroglioma cells, ZsGreen-Vpr shows a localization pattern similar to HEK 293T cells. (A) While ZsGreen protein clearly distributed throughout the intracellular environment, both ZsGreen-Vpr (B) and Vpr-ZsGreen (C) were found mainly at the nuclear envelope, along with localized herniations around the nuclear membrane, and in speckles within the nuclear compartment. First, second, and third columns are DAPI, FITC, and merged views, respectively, and each figure represents a cross-sectional view of the cell. These figures are representative of the entire cell population observed.
Ferrucci et al. Virology Journal 2011 8:397 doi:10.1186/1743-422X-8-397