Localization of ZsGreen-Vpr within U-937 promonocytic cells is predominantly intranuclear. (A) ZsGreen control protein localizes uniformly intracellularly, while both ZsGreen-Vpr (B) and Vpr-ZsGreen (C) are similarly distributed within the nucleus, with a very low level accumulation in the cytoplasm, unlike Jurkat cells. The first, second, and third columns represent DAPI, FITC, and merged views, respectively. All figures denote a cross-sectional view of the cell. Similar results have been obtained in triplicates.
Ferrucci et al. Virology Journal 2011 8:397 doi:10.1186/1743-422X-8-397