ZsGreen-Vpr localizes exclusively in the cytoplasm within Jurkat lymphocytic cells. While control ZsGreen protein (A) was evenly distributed, both ZsGreen-Vpr (B) and Vpr-ZsGreen (C) were found in speckles only within the cytoplasmic compartment, completely excluded from the nucleus. First, second, and third columns are DAPI, FITC, and merged views, respectively. Each figure represents a cross-sectional view of the cell and is representative of the entire cell population observed.
Ferrucci et al. Virology Journal 2011 8:397 doi:10.1186/1743-422X-8-397