Additional file 7.

Figure S6. HCV variants induced incomplete T cell activation and/or up-regulated CD45RBlow Tregs. PBMCs were cultured with peptide NS3358-375 (5 μM) and variant pools (1 μM/each peptide) for 66 hours followed by positive selection of CD4+ T cells with anti-human CD4 antibodies and magnetic beads. The CD4+ cells obtained were then stained with fluorescent-labeled antibodies to human CD134 (OX40), and CD45RB and analyzed using a three-color flow cytometer. PBMCs cultured with medium alone were used as control. The effect of peptide NS3358-375 and variant pools VP1 and VP2 on expression of CD134 (A) and CD45RB (B) on CD4 T cells is demonstrated. A representative of two experiments is shown.

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Wang et al. Virology Journal 2011 8:377   doi:10.1186/1743-422X-8-377