Additional file 4.

Figure S3. Treg phenotypes induced by peptide NS3358-375 and variant pools VP1 and VP2 at about 1.5 years post HCV infection. Cell culture and purification of CD4+ cells and controls were the same as described in Figure 4. Intracellular staining of purified CD4+ cells was performed using fluorescent-labeled antibodies to human CD25, IFN-γ, TGF-β and IL-10. A: Percentages of CD25-IL-10+ Tr1 cells. B: Percentages of CD25+IL-10+ Tr1 cells. A single representative of three experiments is shown.

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Wang et al. Virology Journal 2011 8:377   doi:10.1186/1743-422X-8-377