Figure 3.

Treg phenotypes induced by peptide NS3358-375 and variant pools VP1 and VP2. Cell culture adn purification of CD4+ cells and controls were the same as described in Figure 2. Intracellular staining and four-color flow cytometry using fluorescent-labeled antibodies to human CD25, IFN-γ, TGF-β and IL-10 were performed with purified CD4+ cells. A pool from each test was used as an isotype control. A: Percentages of CD25+ gated CD4+ cells. Note that the percentage of CD25+ cells stimulated by VP2 is significantly higher. B: Percentages of Th3 and Tr1 cells. Note that 58~67% of the CD25+ cells were TGF-β+ (sum of the upper two quadrants). A majority of the CD25+IL-10+ cells were also TGF-β+ (the upper right quadrant). C: Percentages of CD25- gated CD4+ cells. D: Percentages of Th3 and Tr1 cells. Note that the percentages of CD25-TGF-β+ cells stimulated by VP1 and VP2 are significantly higher than stimulation with wild type NS3358-375 and medium alone. Two representatives of eight experiments are shown.

Wang et al. Virology Journal 2011 8:377   doi:10.1186/1743-422X-8-377
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