Effect of E1-A226V mutation on pH dependence of CHIKV induced cell-cell fusion. C6/36 cells infected with LR-GFP-226A or LR-GFP-226V viruses were incubated for 2 min with L-15 medium whose pH was previously adjusted to desired values. The reaction was abrogated by replacement of the fusion medium with 0.5 mL of standard L-15 medium. Percent of fusion was calculated as (1-c/n)x100%, where c is a number of eGFP expressing cells, n is number of nuclei (n≥70). Two independent experiments were performed for each virus (i and ii). Each individual experiment was performed in duplicates and results are expressed as an average of duplicates.
Tsetsarkin et al. Virology Journal 2011 8:376 doi:10.1186/1743-422X-8-376